ISOLATION OF PROTEIN FROM THE SPINE VENOM OF PTEROIS VOLITANS FOUND IN THE INDONESIAN OCEAN, USING A HEATING PROCESS, FOR ANTICANCER, ANTIRETROVIRAL, ANTIBACTERIAL, AND ANTIOXIDANT ASSAYS

Objective: This research investigates the antibacterial, anticancer, antioxidant, and antiretroviral activities of lionfish spine poison extract.
 Methods: Isolation purification phospholipase A2 (PLA2) protein obtained from were conducted through following stages, including, extraction venom by sonication, heating, using gradual saturation levels ammonium sulfate. Furthermore, purity concentration PLA2 analyzed Lowry test Marinetti’s method, respectively, while its content was ascertained SDS-PAGE. Toxicity then evaluated employing brine shrimp lethality (BSLT), anticancer activity assessed in human cervical carcinoma cells (HeLa cells). Finally, DPPH agar diffusion against Salmonella sp. E. coli, SRV-2 RT-qPCR tests, respectively.
 Results: The demonstrated 37.79% inhibition for activity, IC50 1312 ppm antioxidant 98.81%, 89.28% coli respectively antibacterial 98.13% activity.
 Conclusion: It can be concluded that (Pterois volitans) has potential to developed as an agent. pharmacological determined isolating extract, optimum heating temperature 70 °C sulfate level 80%.